Advanced search
Publication Cover
Growth Factors Volume 31, 2013 - Issue 6
Submit an article Journal homepage
129
Views
0
CrossRef citations to date
0
Altmetric
Original Article

Chain-specific antibodies for laminin-511

Laura ZamursThe Ludwig Institute for Cancer Research, Melbourne Branch Parkville, VictoriaAustralia;Department of Surgery, University of Melbourne, Royal Parade, Royal Melbourne Hospital VictoriaAustralia
,
Normand PouliotMetastasis Research Laboratory, Peter MacCallum Cancer Centre, St. Andrews Place East Melbourne, VictoriaAustralia;Sir Peter MacCallum Department of Oncology, University of Melbourne VictoriaAustralia;Pathology Department, University of Melbourne VictoriaAustralia
,
Nicole KusumaMetastasis Research Laboratory, Peter MacCallum Cancer Centre, St. Andrews Place East Melbourne, VictoriaAustralia;Pathology Department, University of Melbourne VictoriaAustralia
,
Edouard C. NiceThe Ludwig Institute for Cancer Research, Melbourne Branch Parkville, VictoriaAustralia;Department of Biochemistry and Molecular Biology, Monash University Clayton, VictoriaAustraliaCorrespondence[email protected]
[email protected]
&
Antony W. BurgessThe Ludwig Institute for Cancer Research, Melbourne Branch Parkville, VictoriaAustralia;Department of Surgery, University of Melbourne, Royal Parade, Royal Melbourne Hospital VictoriaAustralia;The Walter and Eliza Hall Institute of Medical Research Parkville, VictoriaAustraliaCorrespondence[email protected]
Pages 209-219 | Received 17 Oct 2013, Accepted 23 Oct 2013, Published online: 26 Nov 2013
 
Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days

Abstract

Mouse monoclonal antibodies (mAbs) that bind to specific chains of laminin-511 (LM-511) have been developed. Antibody 2F12 binds to the LMα5 chain, 3G10 binds to the LMβ1 chain and 3C12 binds to the LMγ1 chain. These antibodies can be used to purify LM-511, to detect LM-511 in cell extracts or to detect the location of LM-511 in tissue by immunohistochemistry. In combination, the antibodies recognize all three chains of LM-511 and combinations of the antibodies can be used to quantitate levels of LM-511 in physiological fluids. One of the antibodies (3G10) is a potent inhibitor of the activity of LM-511 in cell adhesion, spreading and proliferation assays.

Log in via your institution

Access through your institution

Log in to Taylor & Francis Online

Restore content access

Restore content access for purchases made as guest
Purchase options * Save for later

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 65.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 1,233.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.