Prostaglandin E₂ Levels of Aqueous and Vitreous Humor in Ketorolac 0.4% and Nepafenac 0.1% Administered Healthy Rabbits

ABSTRACT

Purpose: To compare the lowering effects of ketorolac 0.4% and nepafenac 0.1% on aqueous and vitreous humor prostaglandin E₂ (PGE₂) levels in rabbits.

Methods: Ketorolac and nepafenac ophthalmic solutions were administered to the right eyes of 24 healthy rabbits after randomized division into two groups. The left eyes of these rabbits were considered as controls for the two groups. On the 4th day of the experiment, the samples were taken from the aqueous and vitreous humors of the rabbits bilaterally, and PGE₂ levels were measured by an enzyme immune assay kit.

Results: Ketorolac and nepafenac achieved a statistically significant decrease (p<0.001, for each) in PGE₂ levels in the aqueous (11.75 ± 6.15 and 14.75 ± 7.60 pg/mL, respectively) and the vitreous humor (6.58 ± 4.62 and 9.83 ± 4.55 pg/mL, respectively).

Conclusions: Both ketorolac and nepafenac inhibited PGE₂ levels in both the aqueous and vitreous humors of rabbits. Although PGE₂-lowering effects were similar in the aqueous humor, nepafenac seemed to be more potent than ketorolac in the vitreous humor.

Keywords:

• Amfenac
• aqueous humor
• ketorolac
• nepafenac
• PGE₂
• vitreous humor