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Abstract
Background. This study investigated whether follicular fluid (FF) and serum (S) concentrations of cytokines in women undergoing assisted reproductive treatment (ART) were different in GnRH antagonist cycles compared to agonist long ones.
Methods. A retrospective clinical study was performed at a University ART center. A total of 85 women who underwent ART either with agonist long (n = 34) or antagonist protocol (n = 51) were analyzed. FF and serum samples were collected at the time of oocyte retrieval and measured for interleukin (IL)- 1β, IL-6, IL-8, IL-12, tumor necrosis factor (TNF)-α by the enzyme-linked immunosorbent assay (ELISA) technique, using commercially available kits and nitric oxide (NO) by the nitrate/nitrite colorimetric assay. The results were compared between GnRH antagonist and agonist cycles.
Results. No significant difference was found in the FF concentrations of those cytokines between the two protocols. The serum values were also similar in the two groups except IL-6 (14.3 ± 4.8 vs. 20.5 ± 12.2 pg/ml; p = 0.008) and NO (1.4 ± 1.1 vs. 2.2 ± 1.9 μm; p = 0.038) levels which were found to be significantly lower in antagonist cycles.
Conclusions. There is no significant difference in follicular microenvironment in terms of IL-1β, IL-6, IL-8, IL-12, TNF-α, and NO levels between agonist long and antagonist cycles. However, serum IL-6 and NO levels were lower in women given antagonists.
Acknowledgements
The authors thank Prof. Canan Bingol, medical director of Yeditepe University Hospital, Burak Dalan, director of Yeditepe University Hospital, for their support in our research activity, and Prof. Serdar Oztezcan and his team (Yeditepe University Hospital, Department of Biochemistry) for evaluating the samples.