Evaluation of the tissue damage of porcine ovaries after bipolar drilling under transvaginal hydrolaparoscopy – an in vitro experiment

Abstract

Objective. To evaluate the tissue damage of in vitro porcine ovarian drilling in saline solution mimicking transvaginal hydrolaparoscopy (THL).

Study design. In vitro ovarian drilling with different current and activation time was applied on fresh porcine ovaries, 15 ovaries in each group and 2 punctures in each ovary. Drilling using 5-Fr bipolar needle was performed in saline solution mimicking THL, while saline solution rinsing the ovaries after monopolar electrocoagulation.

Results. The monopolar drilling caused more tissue damage than the bipolar needle in saline (p < 0.01). The ratio of the damage of monopolar electrocoagulation (40w, 3s) over that of bipolar diathermy in saline solution (70w, 15s) was 7.4 [(16.74 ± 1.30) mm³/(2.27 ± 0.49) mm³]. In the bipolar groups, the 70w power set (15s and 20s) caused significantly more tissue damage than the 50w ones (p < 0.05), and the amount of damage was not stimulation time dependent (p > 0.05).

Conclusion. The tissue damage caused by monopolar electrocoagulation with 40w for 3s was about seven times of that caused by 5-Fr bipolar one in saline with 70w for 15s . In THL drilling using a 5-Fr bipolar electrode, the current is more crucial than stimulation time.

Keywords:

• Polycystic ovary syndrome
• ovarian drilling
• transvaginal hydrolaparoscopy
• porcine ovary

Acknowledgements

We thank the staff of the department of pathology of Peking University Third Hospital who fixed and sliced the experiment tissue, and Sawaek Weerakiet from Mahidol University, Thailand, who proposed substantive suggestions. This study was supported by Major State Basic Research Development Program of China, No. 2007CB948102.