Abstract
Purpose: To determine whether a dose-response relationship exists among exposure to extremely low frequency magnetic fields (ELF-MF) at different densities and 70-kDa heat shock protein (hsp70) expression and DNA damage in mouse brain.
Materials and methods: Male CD1 mice were exposed to ELF-MF (50 Hz; 0.1, 0.2, 1 or 2 mT) for 7 days (15 h/day) and sacrificed either at the end of exposure or after 24 h. Hsp70 expression was determined in cerebral cortex-striatum, hippocampus and cerebellum by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis. Primary DNA damage was evaluated in the same tissues by comet assay. Sham-exposed mice were used as controls.
Results: No changes in both hsp70 mRNA and corresponding protein occurred following exposure to ELF-MF, except for a weak increase in the mRNA in hippocampus of exposed mice to 0.1 mT ELF-MF. Only mice exposed to 1 or 2 mT and sacrificed immediately after exposure presented DNA strand breaks higher than controls in all the cerebral areas; such DNA breakage reverted to baseline in the mice sacrificed 24 h after exposure.
Conclusions: These data show that high density ELF-MF only induce reversible brain DNA damage while they do not affect hsp70 expression.
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Acknowledgements
We gratefully acknowledge Prof. Ermanno Cardelli and his staff (Dept. of Industrial Engineering, Section of Electrotechnics, University of Perugia) for the useful discussion about the experimental set-up.
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This work was supported by University of Perugia and by Fondazione Cassa di Risparmio di Perugia with the found for Scientific and Technological Research, Grant no. 2006.0274.020.