Gossypetin, a naturally occurring hexahydroxy flavone, ameliorates gamma radiation-mediated DNA damage

Abstract

Purpose: To evaluate the protective effect of gossypetin (GTIN) against gamma (γ)-radiation-mediated DNA damage.

Materials and methods: Increasing concentrations (10–150 μM) of GTIN were incubated with supercoiled DNA 1 h prior exposure to γ-radiation in the range of 5-Gy absorbed dose from Co⁶⁰ γ source. To establish the effective protective concentration of GTIN, supercoiled DNA was pre-incubated with 50 μM of GTIN for 1 h followed by exposure of 5, 10 and 20 Gy doses of γ-radiation. Moreover, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, hydroxyl radical, nitric oxide (NO) scavenging, metal chelating activity and ferric reducing antioxidant power (FRAP) of GTIN were measured and compared with standards. The flowcytometric analysis and radiation-induced genomic DNA damage by comet assay were employed to estimate the level of intracellular reactive oxygen species (ROS) using isolated murine hepatocytes.

Results: GTIN was able to effectively scavenge different free radicals in in vitro situations. It could significantly prevent radiation induced supercoiled and genomic DNA damage with reduced comet parameters. It also acted as a potent scavenger of the radiation induced ROS.

Conclusions: GTIN ameliorated radiation-induced oxidative stress and DNA damage by its free-radical scavenging activity.

Keywords::

• Gossypetin
• gamma radiation
• reactive oxygen species
• DNA damage
• free radical scavenger

Acknowledgements

The authors are thankful to Dr Ajit Kumar Sinha, Centre Director and Dr Abijit Saha of Radiation Chemistry of UGC-DAE CSR, Kolkata Centre, for providing necessary support of irradiation facilities. SD acknowledges CSIR, GOI, for fellowship to KM, WB DBT, Kolkata, India, for fellowship support to DD, CPEPA, UGC, for fellowship to SBK, UGC-DAE for MS.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.