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Abstract
Twenty multi-enzymatic preparations were evaluated for their levels of feruloyl esterase activity and their substrate specificity using methyl ferulate and isolated feruloylated non-digestible oligosaccharides from sugar-beet pulp and wheat bran as substrates. The efficiency of feruloyl esterases expressed in the six best multi-enzymatic preparations from Bacillus spp. (Ceremix), Humicola insolens (Depol 740), Aspergillus oryzae (Flavourzyme), Bacillus amyloliquefaciens (Multifect P 3000), Bacillus subtilis (RP-1) and Trichoderma reesei (Depol 670) for the synthesis of feruloylated glycosides was investigated using a surfactant-less organic microemulsion system as reaction medium. Using n-hexane, 1-butanol and MES (3-(N-morpholino)ethanesulfonic acid)–NaOH buffer reaction mixture (51:46:3 v/v/v), feruloyl esterases expressed in Multifect P 3000 resulted in the highest bioconversion yield of feruloylated arabinose (37%), whereas those present in Depol 670 led to the highest bioconversion yield of feruloylated galactose (61%). The highest bioconversion yield of feruloylated xylose (37%) was obtained using the feruloyl esterase of Depol 670 as biocatalyst in n-hexane, 2-butanone and MES–NaOH buffer reaction mixture (51:46:3 v/v/v). Feruloylated arabinose, galactose and xylose demonstrated potential antioxidant properties as indicated by their profound radical scavenging activity. The chemical structure of the feruloylated glycosides was confirmed by APCI-MS.
Declaration of interest: This project was supported by funds from McGill University and Canadian Foundation for Innovation (CFI). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.