Histological and immunohistochemical studies of the structure of lymph nodes in Kilis goats

Abstract

Ten healthy adult Kilis goat mesenteric lymph nodes were used to examine the general structure of lymph nodes, lymphocytes, plasma cells, reticular cells and reticular fibers using histological methods. We also detected T lymphocytes using anti-CD3 [SP7], anti-CD4 [74-12-4], mouse anti-bovine CD4 [CC30] and mouse anti-bovine CD8 [CC63] monoclonal antibodies (mAb); and B lymphocytes using anti-CD79a [HM57] mAb, macrophages using anti-macrophage [MAC387] mAb and follicular dendritic cells using anti-S100 polyclonal antibody (pAb). The distribution of these cells also was studied. Although the primer antibodies we used for CD3, CD8, CD79a, MAC387 and S100 worked well, the primer antibodies for CD4 were ineffective for paraffin embedded goat lymph nodes.

Key words::

• histology
• immunohistochemistry
• Kilis goat
• lymph node

Acknowledgment

Our study was supported by Mustafa Kemal University Scientific Research Projects (BAP) Coordination Unit.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for its contents.