Measurement of sVEGF R1 and PlGF in Serum: Comparing Prototype Assays from Beckman Coulter, Inc. to R&D Systems Microplate Assays

Abstract

Objective. To compare the performance of prototype Access® sVEGF R1 and PlGF automated immunoassays from Beckman Coulter to the Quantikine® microplate ELISA assays by R&D Systems. Methods. Samples obtained from pregnant women, non-pregnant women and men were assayed according to manufacturers' instructions. Results. Compared to the Quantikine assays, the Access assays demonstrated improved precision, increased sensitivity, broader dynamic ranges, and reduced analysis time. The Access assays were found to be specific for free sVEGF R1 and free PlGF. Conclusion. There was good correlation between the Access and Quantikine assays. Superior performance by Access assays may have important prenatal diagnostic implications.

• Preeclampsia
• Angiogenesis
• Placental Growth Factor
• Soluble Vascular Endothelial Growth Factor
• sFlt-1

ACKNOWLEDGMENTS

We gratefully acknowledge the technical assistance of James Mendoza, Kristen Jones, Patricia Nunnelly, Adele Wen Golden and Adam Ettl. This research was supported (in part) by the Perinatology Research Branch, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, DHHS.

Declaration of Interest

Beckman Coulter provided instrumentation, prototype reagents and financial support to Abbott Northwestern and Toledo Hospitals for this study. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.