Abstract
The associations of serum arginase I with serum L-arginine, serum 3-nitrotyrosine, and fractional exhaled nitric oxide (FENO) were evaluated cross-sectionally in healthy Japanese workers. The serum median (minimum–maximum) levels of arginase I, 3-nitrotyrosine, and FENO in healthy people (n = 130) were 14.6 (0.94–108.1) ng/mL, 81.0 (0.27–298.6) pmol/mg protein, and 14.0 (5.0–110.0) parts per billion, respectively. Significant correlations of arginase I with FENO, L-arginine, 3-nitrotyrosine, and percent predicted forced expiratory volume in 1 s (FEV1 (% predicted)) were observed, and correlations of FENO with immunoglobulin E (IgE), NOx, arginase I, and sex and allergy were also observed. By multiple regression analysis, arginase I showed positive associations with FENO and 3-nitrotyrosine, and a negative association with L-arginine; and FENO showed positive associations with IgE and NO2− + NO3− (NOx), and a negative association with L-arginine, as well as an association with sex. Moreover, logistic regression analysis showed linear inverse associations of arginase I and 3-nitrotyrosine with L-arginine, and showed linear positive associations of FENO with IgE and NOx. It was concluded that serum arginase I might regulate serum L-arginine and 3-nitrotyrosine via L-arginine, and that IgE or NOx might regulate FENO in a healthy Japanese population.
Acknowledgment
The authors thank Professor T. Akaike, Tohoku University, an overseas member of the Japanese Free Radical Society (Oxidative Stress Society), for scientific and critical reading of this manuscript.
Declaration of interest
The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.
This work was supported in part by Grant-in-Aid for Scientific Research No. 19390163 from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government.