Oxidative stress, d-ROMs test, and ceruloplasmin

ABSTRACT

Human serum samples were evaluated for oxidative stress with the d-ROMs test. The ceruloplasmin (CP) and copper contents of the samples was independently measured and compared to those calculated on the basis of the d-ROMs test results for pure CP solutions. The d-ROMs readings did not show any significant correlation with either the CP or copper contents of the samples. Critical interference of CP on the d-ROMs test was therefore excluded and the usefulness of the test in the evaluation of global oxidative status of a biological sample could be reassessed.

KEYWORDS:

• Ceruloplasmin
• copper
• oxidative stress
• radical oxygen metabolites

Notes

¹A list of over one thousand references is available at Diacron International s.r.l.

²Carr units are obtained multiplying by 10⁴ the Optical Density values. They are named after the test inventor.

³When building graphs a, b of Figure 3 and entry 32 of Table 3 was disregarded because the Cu_ICP-MS value was considered affected by a critical error (either instrumental or systemic) being clearly detached from any sensible trend.