Evidence for cytochrome P450 2B1/2B2 isoenzymes in freshly prepared peripheral blood lymphocytes

Abstract

Cytochrome P450 2B1 and 2B2, the major hepatic drug metabolizing enzymes belonging to CYP2 family and associated constitutive androstane receptor (CAR) were found to be expressed in peripheral blood lymphocytes (PBL) isolated from rats. As observed in liver, pretreatment of phenobarbital (PB) or phenytoin were found to increase the expression of CYP2B1, CYP2B2 and associated enzyme activity in PBL. Like in liver, blood lymphocyte CYP2B1/2B2 catalyzed the activity of 7-pentoxyresorufin O-dealkylase (PROD). The present data, demonstrating similarities in the regulation of blood lymphocyte CYP2B-isoenzymes with the liver enzymes, suggests that blood lymphocyte CYP2B-isoenzymes could be used as a biomarker to monitor tissue levels.

Keywords::

• Lymphocyte
• liver
• CYP
• PB
• induction
• expression

Acknowledgments

Authors are grateful to Director, CSIR-Indian Institute of Toxicology Research (IITR), Lucknow for his keen interest in the work. The technical assistance of Mr. B. S. Pandey is also gratefully acknowledged.

Declaration of interest

This work was funded by Indo-US project on Fingerprints of blood Cytochrome P450s: Biomarker of exposure and effect sponsored of ICMR, New Delhi. Mr. Kumar Saurabh is grateful to CSIR, New Delhi, for providing a Senior Research Fellowship. IITR Communication Number: 2928.