Serum metabonomics study of adenine-induced chronic renal failure in rats by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

Abstract

An ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC Q-TOF MS) metabonomics approach was employed to study the serum metabolic profiling of adenine-induced chronic renal failure (CRF) rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the CRF and the normal control groups. Potential biomarkers were screened by using S-plot and were identified by the accurate mass, isotopic pattern and MS/MS fragments information obtained from UPLC Q-TOF MS analysis. Significant differences in the serum level of creatinine, amino acids and LysoPCs were observed, indicating the perturbations of amino acid metabolism and phospholipid metabolism in adenine-induced CRF rats. This research proved that metabonomics is a promising tool for disease research.

Keywords::

• Chronic renal failure
• metabonomics
• serum
• rats
• UPLC Q-TOF MS

Acknowledgements

The authors wish to thank Xu Bai for his expert technical assistance from Waters Technology (Shanghai) Co., Ltd.

Declaration of interest

This study was supported in part by grants from the National Scientific Foundation of China (No. 81001622), project of “As a Major New Drug to Create a Major National Science and Technology Special” (No. 2011ZX09401-308-34) from Ministry of Science and Technology of the People’s Republic of China and Scientific Research Program Funded by Shaanxi Provincial Education Department (No. 11JK0678).