Discovery of biomarkers for oxidative stress based on cellular metabolomics

Abstract

Oxidative stress has a close relationship with various pathologic physiology phenomena and the potential biomarkers of oxidative stress may provide evidence for clinical diagnosis or disease prevention. Metabolomics was employed to identify the potential biomarkers of oxidative stress. High-performance liquid chromatography–diode array detector, mass spectrometry and partial least squares discriminate analysis were used in this study. The 10, 15 and 13 metabolites were considered to discriminate the model group, vitamin E-treated group and l-glutathione-treated group, respectively. Some of them have been identified, namely, malic acid, vitamin C, reduced glutathione and tryptophan. Identification of other potential biomarkers should be conducted and their physiological significance also needs to be elaborated.

Keywords:

• Biomarkers
• metabolomics
• oxidative stress

Disclosure statement

The authors report no conflicts of interest.

Funding information

This research project was financially supported by the National Natural Sciences Foundation of China (21175142 and 21505143), National High Technology Research and Development Program of China (2014AA022203) and Science Foundation of Gansu Province (1308RJYA077).