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Medical Mycology Volume 48, 2010 - Issue 3
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Research Article

Development of a rapid, cost-effective TaqMan Real-Time PCR Assay for identification and differentiation of Coccidioides immitis and Coccidioides posadasii

Kelly W. Sheff *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), Flagstaff
,
Emily R. York *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), Flagstaff
,
Elizabeth M. Driebe *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), Flagstaff
,
Bridget M. Barker †The University of Arizona, Department of Plant Sciences, Tucson
,
Steven D. Rounsley †The University of Arizona, Department of Plant Sciences, Tucson
,
Victor G. Waddell ‡Arizona Department of Health Services, Bureau of State Laboratory Services, Phoenix
,
Stephen M. Beckstrom-Sternberg **TGen North and Northern Arizona University, Center for Microbial Genetics and Genomics
,
James S. Beckstrom-Sternberg §Northern Arizona University, Center for Microbial Genetics and Genomics, Flagstaff, Arizona, USA
,
Paul S. Keim *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), Flagstaff; §Northern Arizona University, Center for Microbial Genetics and Genomics, Flagstaff, Arizona, USA
&
David M. Engelthaler *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), FlagstaffCorrespondence[email protected]
 show all
Pages 466-469 | Received 05 Jun 2009, Accepted 29 Jul 2009, Published online: 06 Apr 2010
 

Abstract

Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

Acknowledgements

This research was supported by grants from the Arizona Biomedical Research Commission (# 0816), Science Foundation Arizona (# CAA0242-08) and the Helios Education Foundation.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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