Abstract
Candida spp. are an important cause of nosocomial bloodstream infections. Currently, complete identification of yeasts with conventional methods takes several days. We report here the first evaluation of an extraction method associated with the Vitek MS matrix-assisted laser desorption ionization time of flight mass spectrometry for direct identification of Candida species from positive blood cultures. We evaluated this protocol with blood cultures that were inoculated with reference and routine isolates (eight reference strains, 30 patients isolates and six mixed cultures containing two strains of different Candida species), or from patients with candidemia (28 isolates). This method performed extremely well (97% correct identification) with blood cultures of single Candida spp. and significantly reduced the time of diagnosis. Nevertheless, subculture remains indispensable to test fungal resistance and to detect mixed infections.
Acknowledgments
We gratefully acknowledge all the technicians of the morphology unit of the department of Parasitology Mycology for the strain culture, John Woodley for the English revision of the manuscript, and Damien Dubois and bioMérieux SA for their technical assistance.
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and the writing of the paper.