Abstract
Pneumocystis jirovecii, a transmissible fungus, is the causative agent of pulmonary infections. Its genomic diversity has appeared in reports from around the world but data on P. jirovecii genotypes in France are still limited. This study describes the typing of P. jirovecii isolates from 81 HIV-negative patients monitored at Brest University Hospital, Brittany, France, 40 of whom developed Pneumocystis pneumonia (PcP), and remaining 41 patients were colonized by the fungus. The isolates were assayed at the internal transcribed spacer (ITS)1 and ITS2 under improved amplification conditions to avoid in vitro ITS recombination. P. jirovecii ITS haplotypes were identified in 56/81 patients (31 PcP patients and 25 patients who were colonized) which revealed a high diversity in that 27 different haplotypes were identified. Eg was the most frequent haplotype (31/56, 55.3%), followed by Ec and Ai (5/56, 8.9% each). In contrast, Ne, usually the second most frequent haplotype in Europe and the USA, was observed in only 2/56 patients (3.6%). Mixed infections were detected in 18/56 patients (32.1%; 12 PcP patients and six who were colonized). No significant differences were observed in haplotype diversity, frequency of peculiar haplotypes, and mixed infection occurrence, between the two patient populations. The study, conducted with the largest HIV-negative patient population investigated so far, shows that ITS typing remains an efficient method for characterizing P. jirovecii among human populations, whatever their clinical presentation of Pneumocystis infections.
Acknowledgments
The authors would like to thank the following physicians: Drs S. Airiau, N. André, S. Ansart, A. Barnier, A. Cleuziou, F. Dalbies, A. Delluc, H. Floch, M. Garré, A. Gentric, K. Goulesque A. Grall, C. Gut-Gobert, C. Hanrotel-Saliou, E. Huchot, S. Jaffuel, K. Lacut, R. Le Berre, P. Le Magnet, E. Le Moigne, C. Leroyer, M.C. Moal, O. Pradier, G. Prat, G. Quéré, A. Renault, N. Rouzic, L. de Saint Martin, A. Saraux and C. Verveur for monitoring the patients from whom P. jirovecii specimens were obtained. The authors would also like to thank Dr D. Quinio and Dr E. Moalic, and Mrs P. Lecordier, C. Carrou, D. Roué, C. Le Guen, C. Roger, C. Cam, K. Quinaou and V. Abiven for performing biological diagnoses of Pneumocystis infections.
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and the writing of the paper.
This study was supported in part by the ‘Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (Anses)’ (contract number 2011/1/053).
This paper was first published online on Early Online on 22 August 2013.