Physical training normalizes nucleotide hydrolysis and biochemical parameters in blood serum from streptozotocin-diabetic rats

Abstract

Ectonucleotidases and the nucleotide metabolism have been implicated as important regulators in diabetes disease. We evaluated the ectonucleotidase activities and biochemical parameters in blood serum of streptozotocin (STZ)-induced diabetic rats submitted a physical training protocol. We observed a raise in ATP, ADP, AMP and p-Nph-5′-TMP hydrolysis rate and in the levels of cholesterol and triglycerides in rat blood serum, after 30 days of diabetes induction. However, in serum of rats submitted a physical training protocol by forced swimming, both the nucleotide hydrolysis rate and the lipids levels returned to the control values. Considering that diabetes leads to multiple pathophysiological alterations, the modulations observed in ectonucleotidase activities may be part of the events involved in these alterations. Then the physical training is a very important way to control the vascular alterations developed in diabetes.

Keywords::

• Diabetes
• physical training
• E-NTPDases
• ecto-5′-nucleotidase/CD73
• E-NPPs

Acknowledgements

We are grateful to the veterinarian Simone Gerson for her fundamental assistance assuring the high quality for animal care.

Declaration of interest

IPA Methodist University Center was responsible for the financial support for this study. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.