Abstract
Background aims. Mesenchymal stromal cells (MSC) are the most popular cells used in regenerative medicine and biotechnology. The clonogenic potential of these cells is defined by colony-forming unit-fibroblasts (CFU-F). It is well known that there is an interaction between hematopoietic cells and stromal cells in disease formation pathogenesis. Therefore we hypothesized that there should be a quantitative and qualitative relationship between MSC colonies (CFU-F) and hematopoietic stem cell colonies (colony-forming unit-granulocyte-macrophages; CFU-GM) among patients with and without hematologic diseases. Methods. Forty-two patients were included in this study. Patients were divided into three groups: group A, patients with hematologic malignancies (n =20); group B, patients with bone marrow (BM) failure (n =11); group C, patients without hematologic diseases (n =11). BM aspirates were plated in different densities for CFU-F culture. The plating density was the same for CFU-GM culture. Results. CFU-GM colonies grew in 90% of group A cells and all of group B and C cells (P= 0.0001). CFU-F colonies became visible on the ninth day of plating in group A and on the eight day in groups B and C. There was no statistically significant difference between the groups for the duration of CFU-F colony formation (P= 0.12). There were differences in the morphology of the colonies among the groups. Conclusions. This is the first study that has compared the clonogenic potential of stromal cells and hematopoietic stem cells in the same subjects with and without hematologic diseases. No correlation was shown between the clonogenic potential of stromal cells and hematopoietic cells.
Acknowledgments
We thank Associate Profesor Ömer Koç and his laboratory team, Emese Szekely, Bashabi Maitra and Mary Groh (Case Western Reserve University, Ohio, USA), for teaching us plating and passaging of MSC, and sharing their knowledge with us.
This project was supported by the Scientific Research Projects Office and Biotechnology Institute of Ankara University (Scientific Research Project Number 40809182).
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.