Abstract
Dendritic cells (DC) are professional antigen-presenting cells currently being used as a cellular adjuvant in cancer immunotherapy strategies. Unfortunately, DC-based vaccines have not demonstrated spectacular clinical results. DC loading with tumor antigens and DC differentiation and activation still require optimization. An alternative technique for providing antigens to DC consists of the direct fusion of dendritic cells with tumor cells. These resulting hybrid cells may express both major histocompatibility complex (MHC) class I and II molecules associated with tumor antigens and the appropriate co-stimulatory molecules required for T-cell activation. Initially tested in animal models, this approach has now been evaluated in clinical trials, although with limited success. We summarize and discuss the results from the animal studies and first clinical trials. We also present a new approach to inducing hybrid formation by expression of viral fusogenic membrane glycoproteins.
Acknowledgments
We thank Nicolas Larmonier and Karla Martinez Del Cueto for careful reading and helpful discussions. This work was supported by the Burgundy committee of the French National League against Cancer. DC received grants from the Saône-et-Loire committee of the French National League against Cancer and from the French Association for Cancer Research (ARC).
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.