Aldehyde dehydrogenase as an alternative to enumeration of total and viable CD34⁺ cells in autologous hematopoietic progenitor cell transplantation

Abstract

We validated the correlation of aldehyde dehydrogenase ALDH^br cells with total and viable CD34⁺ cells in fresh and thawed hematopoietic progenitor cell (HPC) products, and looked for a correlation with time to white blood cell (WBC) and platelet engraftment after autologous transplantation, using simple linear regression analyzes. We found a significant correlation between pre-freeze ALDH^br cell numbers and pre-freeze total CD34⁺ (P < 0.001), viable CD34⁺ (P < 0.001) and post-thaw viable CD34⁺ (P < 0.001) cell numbers. We suggest that ALDH^br may be substituted for CD34⁺ cell numbers when evaluating HPC. As post-thaw viability testing apparently adds no significant information, we suggest that it may not be necessary. Finally, neither marker correlated with time to engraftment in our patients, supporting previous data suggesting the existence of a threshold dose for timely engraftment around 2.5 × 10⁶ cells/kg.

Key Words::

• aldehyde dehydrogenase
• CD34
• engraftment
• transplant
• viability

Conflicts of interest: The authors declare that they have no conflicts of interest relevant to the manuscript.

Source of support: None.