Abstract
Objective: To determine amniotic fluid total antioxidant capacity (TAC), ferric-reducing antioxidant power (FRAP) and thiobarbituric acid-reacting substances (TBARS), markers of oxidative stress, in pregnancies complicated by preterm prelabor rupture of membranes (pPROM) and their correlation to microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA).
Methods: One-hundred thirty-eight women with singleton pregnancies complicated by pPROM were included in this study. Amniotic fluid was collected by transabdominal amniocentesis at the time of admission and amniotic fluid concentrations of TAC, FRAP and TBARS were measured.
Result: The presence of MIAC and/or HCA did not show any significant differences in the amniotic fluid TAC, FRAP and TBARS concentrations. Positive correlations between gestational age at sampling and amniotic fluid TAC and FRAP concentrations were found (TAC: rho = 0.32; p = 0.0002; FRAP: rho = 0.36; p < 0.0001). A negative correlation between gestation age at sampling and amniotic fluid TBARS concentrations was identified (rho = –0.25; p = 0.004).
Conclusions: Oxidative stress is associated with pPROM as indicated by the presence of markers tested in the amniotic fluid; however, oxidative stress markers tested are not influenced by the presence of MIAC or HCA.
Declaration of interest
The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.
This work was supported by grant from the Ministry of Health, Czech Republic (NT13461-4/2012) and by REVOGENE project, ITMS 26240220067, supported by the Research & Development Operational Program funded by ERDF. Additional support came from Charles University in Prague, the Faculty of Medicine in Hradec Kralove, Czech Republic, project “PRVOUK” P37/10, the Faculty Hospital in Hradec Kralove (a long-term organization development plan).