Investigation of the effects of 50 Hz magnetic fields on platelet aggregation using a modified aggregometer

Abstract

Purpose: Electromagnetic fields have various effects on intracellular calcium levels, free oxygen radicals and various enzymes. The platelet activation pathway involves an increase in intracellular calcium levels and protein kinase C activation; and free oxygen radicals play a mediating role in this pathway. This study investigated whether 1 mT and 6 mT, 50 Hz magnetic fields had any effects on platelet aggregation.

Materials and Methods: Blood from healthy volunteers was anticoagulated with either citrate or heparin. Each sample was divided in half and assigned to exposure and control groups. Platelet rich plasma samples in the exposure group were exposed to a 1 mT or a 6 mT, 50 Hz magnetic field for 1.5 or 1 h, respectively. The samples from both exposure and control groups were simultaneously evaluated using a modified optical aggregometer. Adenosine-diphosphate, collagen, and epinephrine were used as inducing agents. The slopes of the aggregation curve, the maximum values and the areas under the curves were recorded and compared.

Results: A significant effect was observed only in the 1 mT-citrate group. It was found that magnetic field exposure significantly increased the maximum values and slopes of the collagen-induced aggregations.

Conclusions: It was found that magnetic field exposure has an activating effect on platelet aggregation.

Keywords::

• Electromagnetic Fields
• Platelet Aggregation
• Optical Aggregometer

Acknowledgements

This project was supported by Uludağ University Scientific Research Unit (Project Number: T-2008/47) and Ege University Committee for Scientific Research Projects (Project Number: 2008-TIP-013).

Declaration of Interest The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.