Combined effects of flow-induced shear stress and electromagnetic field on neural differentiation of mesenchymal stem cells

ABSTRACT

Differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into neural phenotype has been induced by either flow-induced shear stress (FSS) or electromagnetic fields (EMF). However, procedures are still expensive and time consuming. In the present work, induction for 1 h with the combination of both forces showed the presence of the neural precursor nestin as early as 9 h in culture after treatment and this result lasted for the following 6 d. In conclusion, the use of a combination of FSS and EMF for a short-time renders in neurite-like cells, although further investigation is required to analyze cell functionality.

KEYWORDS:

• Extremely low-frequency electromagnetic fields
• fluid shear stress
• bone marrow-derived mesenchymal stem cells

Acknowledgments

The authors thank MSc Juan Eduardo Sosa Hernández for the image analysis.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.