Two Different Pulsed Elf Waveforms Affect Rat Liver Regeneration

Abstract

This study aimed to determine the effect of two ELF-PEMFs with different waveforms on rat liver regeneration following partial hepatectomy. The shape of one pulse was a major sinusoidal halfwave (50 Hz, 6 mT) followed by a few cycles of different frequency (400 Hz) and intensity (0.6 mT). The shape of the other pulse was a positive triangle (50 Hz, 8 mT peak) followed by a pause of 2 ms. We have investigated two early phenomena of rat liver regeneration, i.e., glycogen depletion and lipid accumulation by means of electron microscopy and stereological analysis. Since folates are centrally involved in some biosynthetic pathways fundamental to cell replication, we have also studied the incorporation and distribution of [³H]-folk acid into the hepatic cytosolic binders by means of biochemical determinations. Our data show that the two PEMFs have the same biological effect, confirming that treatment with ELF-PEMFs enhances the rate of liver regeneration following partial hepatectomy.