Surface functionalization affects the zeta potential, coronal stability and membranolytic activity of polymeric nanoparticles

Abstract

Nano materials are commonly functionalized to boost their physicochemical properties. However, there is little known about the impact of these modifications on cellular systems. Herein, we synthesized eight types of polymeric nanoparticles (NPs) bearing different functional groups, and investigated their effects on interactions with cellular membranes. As models for particle membrane interactions, hemolysis assays using human red blood cells and culture with A549 cells were utilized. Under protein-free conditions, the NPs showed a wide distribution of zeta potentials (ζPs) which showed a good correlation with their hemolytic potential. However, in the presence of serum or lung lining fluid, the ζPs of all NPs coalesced towards a single common negative value and showed neither hemolytic activity nor cytotoxicity to A549 cells. Lipase and protease treatment of the coronated particles did not restore their reactivity. These result simply proves that particle functionalization influences the stability of the particle corona which, if intact, prevents hemolytic activity and membrane disrupture.

Keywords::

• corona
• hemolysis
• polymeric nanoparticles
• surface functionalization
• toxicity
• zeta potential

Acknowledgments

We gratefully acknowledge the financial support provided by the Medical Research Council of the United Kingdom (MRC G0701323; W-S.C., F.T.), 13182KFDA606 (W.-S.C.), Novartis Foundation (E.M.V.J.) the Natural Environment Research Council (NE/E007791/1; K.D.) and the Colt Foundation (K.D.).