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Abstract
Monotrete leiurus is the only freshwater puffer founded in Nala River, which is a tributary of the Langcang–Mekong River in China. The complete mitochondrial genome sequence of the Monotrete leiurus is 16,448 bp long and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region (CR). The base composition of the genome is 29% A, 23.8% T, 31.8% C and 15.4% G and this composition shows a bias for A + T. The mitochondrial genes from Monotrete leiurus are overlapped in a total of 34 bp at seven locations and interleaved with a total of 64 bp intergenic spacers at 13 locations. The results can not only help clarify the classification problem in sibling freshwater puffers, but also provide information for their protection in the future.
Acknowledgements
The authors are grateful to Mr. Xin Huang and Miss Baojuan Yang and Liqian Ren for their help in the lab work and specimen preparation.
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing style of this article. This study was funded by the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSCX2-EW-J-2) and the National Natural Science Foundation of China (U0936602).