The complete mitochondrial genome of the hybrid of Megalobrama amblycephala (♀) × Parabramis pekinesis (♂)

Abstract

The complete mitochondrial genome sequence of the hybrid of blunt snout bream (Megalobrama amblycephala) (♀) × white Amur bream (Parabramis pekinesis) (♂) (M × P) was determined using the PCR-based method for the first time. The total length of the mitogenome was 16,623 bp in accordance with blunt snout bream. It contained 37 genes (13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes) and 2 main non-coding regions (the control region and the origin of the light strand replication). Compared with the complete mitochondrial genome of the parents, results showed the hybrid bream was consistent with a maternal inheritance; however, we also found a termination codon mutation in Cytb and a double-based site in 16S rRNA which was species-specific for the male parent P. pekinesis. The complete mitochondrial genome sequence of the M × P bream provided an important data set for further study in mitochondrial inheritance mechanism.

• 16S rRNA
• Cytb
• hybrid of Megalobrama amblycephala (♀) × Parabramis pekinesis (♂)
• maternal inheritance
• mitochondrial genome

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This work was financially supported by Modern Agriculture Industry Technology System Construction Projects of China titled as—Staple Freshwater Fishes Industry Technology System (No. CARS-46-05), the National Natural Science Foundation of China (No. 31201988), Projects of Hubei Cooperation and Exchanges (No. 2013BHE006), Wuhan Engineering Research Center of Breeding for Wuchang Bream and Fundamental Research Funds for the Central Universities (No. 2013PY066).