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Abstract
The complete mitochondrial genome sequence of Great tit Parus major was sequenced used polymerase chain reaction (PCR), long-and-accurate PCR and directly sequencing by primer walking. The Genbank accession was KP137624. The entire mitochondrial genome of P. major is a circular molecule of 16,776 bp in length and the content of A, T, C and G were 29.68%, 22.63%, 33.56% and 14.13%, respectively. The complete mitochondrial genome of P. major contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, plus 1 control regions and was similar to most of the other Aves birds in gene arrangement and composition. The complete mitochondrial genome of P. major could provide a useful data for resolving phylogenetic relationship problems related to Parus and P. major subspecies complex.
Declaration of interest
The authors report no conflict of interest. The authors alone are responsible for the content and writing of the article. This work was supported by the National Natural Science Foundation of China (31301889), the Ph.D. Programs Foundation of Ministry of Education of China (20120211120040) and the National Science Foundation for Fostering Talents in Basic Research of the National Natural Science Foundation of China (J1210077, J1210033).