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Mitochondrial DNA Part A
DNA Mapping, Sequencing, and Analysis
Volume 27, 2016 - Issue 1
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Mitogenome Announcement

The complete mitochondrial genomes of two chiton species (Sypharochiton pelliserpentis and Sypharochiton sinclairi) obtained using Illumina next generation sequencing

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Pages 537-538 | Received 06 Mar 2014, Accepted 15 Mar 2014, Published online: 08 Apr 2014
 

Abstract

Using an Illumina platform, we shot-gun sequenced the complete mitochondrial genomes of two sister chiton species (Sypharochiton pelliserpentis and Sypharochiton sinclairi) to an average coverage of 172× and 60×, respectively. We performed a de novo assembly using SOAPdenovo2 and determined the total mitogenome lengths to be 15,048 and 15,028 bps, respectively. The gene organization was similar to that of other chitons, with 13 protein-coding genes, 24 transfer RNAs and 2 ribosomal RNAs. These data will contribute for resolving the taxonomy and population genetic structures of these species.

Acknowledgements

Authors thank New Zealand Genomics Ltd; the Centre for Genomics, Proteomics and Metabolomics.

Declaration of interest

This study was supported by the University of Auckland.

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