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Mitochondrial DNA Part A
DNA Mapping, Sequencing, and Analysis
Volume 27, 2016 - Issue 3
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Mitogenome Announcement

The complete mitochondrial genome of Holland’s spinibarbel Spinibarbus hollandi Oshima (Cypriniformes, Cyprinidae)

, , &
Pages 1721-1723 | Received 28 Aug 2014, Accepted 31 Aug 2014, Published online: 22 Sep 2014
 

Abstract

In this study, the complete mitogenome sequence of cyprinid fish, Holland’s spinibarbel Spinibarbus hollandi Oshima (1919) (Cypriniformes, Cyprinidae, Barbinae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,523 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes, and a non-coding control region (CR). CR of 867 bp length is located between tRNAPro and tRNAPhe. The overall base composition of S. hollandi is 31.87% for A, 26.56% for C, 25.89% for T, and 15.68% for G, with a slight AT bias of 57.76%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.

Declaration of interest

The authors declare no competing materials in the preparation and execution of this manuscript. The authors are responsible for the content and writing of this paper. The corresponding author (ISC) wishes to thank for the grant support for aquatic biodiversity research from CEO, NTOU. The current work was also supported from the Taiwan-Russia international project grants of National Science Council, Taipei for the corresponding author.

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