Abstract
The complete mitochondrial DNA of an ecologically important crustacean mud shrimp, Upogebia yokoyai (Decapoda, Crustacea) was sequenced. We used next generation sequencing strategy for total genomic DNA and organelle genome pipeline for mitogenome assembly. A newly determined mitogenome was 16,063 bp in total length with 28% of GC content. Thirty-seven genes were identified including 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. We found ten case of overlapping between neighboring genes. Based on genome comparison, the mitogenome of U. yokoyai shows general crustacean gene content and identical synteny to the sister species, such as U. major and U. pusilla. Our results will provide useful information for mitochondrial genome diversity and evolution of the Crustacea.
Acknowledgements
We thank Mr. Sang Heon Lee for the sample collection and Ms. Sujeong Lee for molecular work.
Declaration of interest
This work was supported by grants from the Korea Institute of Ocean Science & Technology (PE99195) to JHN and the Basic Science Research Program through the National Research Foundation of Korea (NRF) founded by the Ministry of Science, ICT and Future Planning (2014R1A1A1006512) to ECY. The authors report no conflicts of interest. The authors are responsible for the content and writing of the paper.