Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis

Abstract

Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC – 0.258), band informativeness (∑I_b – 3.283) and marker index (MI – 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.

• Haplogroups
• marker index
• mitochondrial DNA
• PCR-RFLP
• polymorphism

Acknowledgements

We thank all of the sample donors for their voluntary support. The authors thank ImtimenlaJamir, YangerdenlaJamir, Amenti, ThungdenoShitiri, Dimple Saikia, Sikha Morang, Ningsangwabang, Hollohon K Tolhopu and Hanumant Singh Rathore from the School of Engineering and Technology, Nagaland University, Dimapur, India.

Declaration of interest

The authors declare no competing interest. This work was supported by the State Biotech Hub and Bioinformatics Infrastructure Facility sponsored by the Department of Biotechnology (DBT), New Delhi, India.