Complete sequence and characterization of mitochondrial and chloroplast genome of Chlorella variabilis NC64A

Abstract

The complete nucleotide sequences of the mitochondrial (mtDNA) and chloroplast (cpDNA) genomes of Chlorella variabilis NC64A (Trebouxiophyceae) have been determined in this study (GenBank accession no. KP271968 and KP271969, respectively). The mt genome assembles as a circle of 78,500 bp and contains 62 genes, including 32 protein-coding, 27 tRNA and 3 rRNA genes. The overall GC content is 28.2%, while the coding sequence is 34%. The cp genome forms a circle of 124,793 bp, containing 114 genes, including 79 protein-coding, 32 tRNA and 3 rRNA genes. The overall GC content is 33,9%, while the coding sequence is 50%.

• Chlorella variabilis
• Chlorellaceae
• Chloroplast genome
• DNA sequencing
• Mitochondrial genome

Declaration of interest

The authors report no conflicts of interest. The financial support of Department of Mechanical, Chemical and Materials Engineering, University of Cagliari, CRS4 and Sardegna Ricerche is gratefully acknowledged. C. C. and V. M. gratefully acknowledge the Sardinia Regional Government for the financial support of the Scholarship awarded to attend the International PhD program in Environmental Science and Engineering at the University of Cagliari and the awarded grant INNOVA.RE, WP 2.3-P.O.R.-F.E.S.R. 2007/2013- CUP: F25C10001420008, respectively. We thank Professor James L. Van Etten (University of Nebraska, USA) for providing culture of Chlorella variabilis NC64A and Dr James R. Gurnon (University of Nebraska, USA) for the cultivation instructions received.