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Abstract
The complete mitogenome of Polydactylus sextarius was firstly determined in this study using a long polymerase chain reaction (PCR) method and genome walking. It is 16,836 bp in length and has 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region. The genes composition and order of which are similar to most other vertebrates. All mitochondrial genes are encoded on the heavy strand, except for eight tRNA and ND6 genes. The overall base composition of the heavy strand is 27.4% A, 26.0% T, 29.9% C, and 16.7% G, with a slight AT bias of 53.4%. There are eight regions of gene overlap totaling 27 bp and 16 intergenic spacer regions totaling 344 bp. The mitogenome sequence of P. sextarius could contribute to the solution of its phylogenetic position within Polynemidae fishes based on the whole mitogenomic data.
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This study was supported by the Science and Technology Project of Zhejiang Province (2014F50003) and the Special Major Science and Technology Program of Zhejiang Province (2012C13005).