Abstract
Heteroplasmy due to length polymorphism with tandem repeats in mtDNAs within individual was hardly studied in domestic animals. In the present study, we identified intra-individual length variation in the control region of mtDNAs in Nepalese sheep by molecular cloning and sequencing techniques. We observed one to four tandem repeats of a 75-bp nucleotide sequences in the mtDNA control region in 45% of the total Nepalese sheep sampled in contrast to the Chinese sheep, indicating that the heteroplasmy is specific to Nepalese sheep. The high rate of heteroplasmy in Nepalese sheep could be a resultant of the mtDNA mutation and independent segregation at intra-individual level or a strand slippage and mispairing during the replication.
Acknowledgements
The authors wish to thank the staff of Animal Breeding Division for their assistance in collecting samples.
Declaration of interest
The authors report no conflicts of interests. The authors alone are responsible for the content and writing of this article. This work was supported by the National Natural Science Foundation of China (31272403) and the Agricultural Science and Technology Innovation Program of China (ASTIP-IAS01).