Mitochondrial genome sequences of landsnails Aegista diversifamilia and Dolicheulota formosensis (Gastropoda: Pulmonata: Stylommatophora)

Abstract

The first mitochondrial genome sequences of Aegista and Dolicheulota belonging to Bradybaenidae are described in this report. Mitogenomic sequences were generated from Illumina paired-end sequencing. The complete mitogenome of Aegista diversifamilia was 14,039 bp in length and nearly complete mitogenome of Dolicheulota formosensis was 14,237 bp. Both mitogenomes consisted of 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22 transfer RNA genes. Most genes were overlapped with neighboring genes that the overlapping regions ranged from 2 to 64 bp in A. diversifamilia and from 1 to 45 bp in D. formosensis. Novel gene arrangement, tRNA–Tyr–ND3–tRNA–Trp, was identified in A. diversifamilia, whereas D. formosensis showed identical gene order to other Bradybaenidae mitogenomes. Maximum likelihood phylogenetic tree suggested Aegista as a sister clade to Euhadra and Dolicheulota. Bradybaenidae is monophyly sister clade to Camaenidae.

• Bradybaenidae
• Helicoidea
• mitogenome
• Taiwan

Acknowledgements

We would like to thank Genomics BioSci & Tech (Taiwan) for the discussion of experimental design. Thanks for BGI (Shenzhen, China) for the service of sequencing.

Declaration of interest

This work was partially supported by the Center for Information Technology Innovation and Biodiversity Research Center, Academia Sinica. The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of this article.