The first mitochondrial genome of Coelomactra antiquata (Mollusca: Veneroida: Mactridae) from Guangxi (China) and potential molecular markers

Abstract

The complete mitochondrial genome of Coelomactra antiquata (Guangxi, in China, GX) was determined. It is 16 801 bp in length and is the first representative from this province. The mitochondrial genome encodes 35 genes, including 12 PCGs, two ribosomal RNA, and 21 transfer RNA genes. Atp8 and trnSer(UCN) genes are missing, compared with the typical gene content of animal mitochondrial genomes. Three (cob, nad1, nad4, and nad6) of the 12 PCGs in the mitochondrial genome initiate with the ATA, while other PCGs start with ATG. Two PCGs (atp6 and nad4L) end with incomplete stop codons (T–), and the remaining ones have complete stop codons (TAA or TAG). The largest non-coding region of the C. antiquata (GX) contains one section of tandem repeats (5 × 99 bp). Among all PCGs and rRNAs, the nad5 gene contains the maximum polymorphic sites (430), followed by nad4 (261) and cox2 (240). Two ribosomal RNA genes (srRNA and lrRNA) and cox1 are most conservative. The proportions of polymorphic sites in six genes (nad4, nad2, nad6, nad5, cox2, and nad3) are more than 20% (ranging from 20.25% to 25.21%). These high variable genes can be used as molecular markers in the population genetic analysis of the species.

• Coelomactra antiquata
• Mactridae
• mitochondrial genome
• molecular marker
• Mollusca
• Veneroida

Acknowledgements

The authors greatly thank Prof. Ka Hou Chu (Chinese University of Hong Kong, CUHK) for crucial comments.

Declaration of interest

This work was sponsored by Jiangsu Natural Science Funds (BK20131210), Open-end Fund of Jiangsu Key Laboratory of Marine Biotechnology (2011HS009 and 2014HS003), projects funded by the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions (KYCX12 and KYCX14), Huaihai Institute of Technology Natural Science Fund (Z2014019), Open-end Fund of Jiangsu Institute of Marine Resources (JSIMR201411), Jiangsu 333 and Lianyungang 521 Talent Projects. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.