Shotgun assembly of the first mitochondrial genome of Metapenaeus (Metapenaeus ensis) with phylogenetic consideration

Abstract

The first complete mitochondrial genome sequence of Metapenaeus was characterized from sand shrimp Metapenaeus ensis using shotgun assembly method. The complete mitochondrial DNA sequence is a 15 944 bp circular molecule, and contains 13 protein-coding genes, 22 transfer RNA genes, two rRNA genes, and a control region. The gene arrangements are consistent with the pancrustacean ground pattern. The phylogenetic trees were divided two clades besides outgroup, which belonged to Penaeus s.l. and Metapenaeus genus. The molecular analyses provided robust evidence for the monophyly of Fenneropenaeus, but Litopenaeus is not monophyletic.

• Mitochondrial genome
• Metapenaeus ensis
• shrimp
• shotgun assembly

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This work was supported by the Special Scientific Research Funds for Central Non-profit Institute, Chinese Academy of Fishery sciences (2014A11JC03), the Science and Technology Infrastructure Construction Project of Guangdong Province (2013B060400014; 2014A030305005), the National Basic Research Special Foundation of China (2013FY110700), the National Infrastructure of Fishery Germplasm Resource Project (2015DKA30470), and the Financial Fund of the Ministry Agriculture (NFZX2013).