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Abstract
In this study, we sequenced the complete mitochondrial genome of the hybrid of Megalobrama skolkovii (♀) × Megalobrama amblycephala (♂) for the first time. The complete mitochondrial genome of the hybrid bream was found to be 16 621 bp in size with a mostly conserved structural organization when compared with that of other Megalobrama species. It contained 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and two main non-coding regions (the control region and the origin of the light strand replication). Sequence alignment of mitochondrial genomes between the hybrid and its female parent showed that a total of 38 mutation sites in 13 genes or regions, in particular, three sense mutations in three protein-coding genes (COX1, ND4L, and ND5) with 27 mutation sites in nine protein-coding genes. This mitogenome sequence data would contribute to a better understanding of genetic mechanisms of mitochondrial DNA and phylogenetic analysis in hybrids.
Declaration of interest
The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This work was financially supported by National Natural Science Foundation of China (No. 31472271), Modern Agriculture Industry Technology System Construction Projects of China titled as-Staple Freshwater Fishes Industry Technology System (No. CARS-46-05), Excellent Youth Foundation of Hubei Scientific Committee (No. 2013CFA032) and Fundamental Research Funds for the Central Universities (No. 2014PY013).