It has come to the attention of the Editorial Staff that was erroneously printed in Virulence Volume 1, Issue 2 in manuscript Jones MB, Peterson SN, Benn R, Braisted JC, Jarrahi B, Shatzkes K, et al. Role of luxS in Bacillus anthracis growth and virulence factor expression. Virulence 2010; 1:72–83. was omitted during final submission.
The correct for this manuscript is published here:
Figure 1 Induction of bioluminescence in V. harveyi reporter strain by CFM from B. anthracis cells. V. harveyi strain BB170 is deficient in the AI-1 sensor encoded by luxN, and thus only upregulates the expression of the lux operon [measured as relative light units (RLU)], when AI-2 or AI-2-like molecules are present in its milieu. Cell-free medium (CFM) obtained from AI-2-synthesizing bacteria grown to high density (including BB170 in which the AI-2-regulated system is intact) can induce expression of the bioluminescence-generating luxCDABE operon in BB170. (A) In the experiments shown, CFM from 5 h cultures of B. anthracis strains 34F2 and 34F2ΔluxS and sterile CFM alone were used as positive and negative controls. Cells of BB170 were grown for 5 h in the presence of sterile CFM. The baseline is the value when uninoculated (sterile) CFM alone at 5 h were used. Each bar represents the mean (±SD) of triplicate experiments. Compared to the negative and positive controls, 34F2ΔluxS:comp showed restored AI-2 activity compared to wild-type 34F2. (B) In the experiment shown, CFM from 5 h cultures of wild-type 34F2 and 34F2ΔluxS:comp were serially diluted 1:1, 1:10 and 1:100. Each bar represents the mean (±SD) of triplicate experiments.
![Figure 1 Induction of bioluminescence in V. harveyi reporter strain by CFM from B. anthracis cells. V. harveyi strain BB170 is deficient in the AI-1 sensor encoded by luxN, and thus only upregulates the expression of the lux operon [measured as relative light units (RLU)], when AI-2 or AI-2-like molecules are present in its milieu. Cell-free medium (CFM) obtained from AI-2-synthesizing bacteria grown to high density (including BB170 in which the AI-2-regulated system is intact) can induce expression of the bioluminescence-generating luxCDABE operon in BB170. (A) In the experiments shown, CFM from 5 h cultures of B. anthracis strains 34F2 and 34F2ΔluxS and sterile CFM alone were used as positive and negative controls. Cells of BB170 were grown for 5 h in the presence of sterile CFM. The baseline is the value when uninoculated (sterile) CFM alone at 5 h were used. Each bar represents the mean (±SD) of triplicate experiments. Compared to the negative and positive controls, 34F2ΔluxS:comp showed restored AI-2 activity compared to wild-type 34F2. (B) In the experiment shown, CFM from 5 h cultures of wild-type 34F2 and 34F2ΔluxS:comp were serially diluted 1:1, 1:10 and 1:100. Each bar represents the mean (±SD) of triplicate experiments.](/cms/asset/63119631-b95d-40d8-bbca-87a1e67b8bb0/kvir_a_10915095_f0001.gif)