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Abstract
We previously showed that methylglyoxal-induced glycation induces adipose tissue lesions, including decreased irrigation and macrophage recruitment, independently of obesity. Here, we developed a model of partially decreased adipose tissue irrigation, a common condition in obese individuals. We aimed to study the role of methylglyoxal in the metabolic adaptations to such conditions 1 and 48 hours after decreased blood supply, avoiding other confoundable variables. Irrigation decrease during 1 hour leaded to increased activation of ERK1/2 and degradation of Ikappa-Balpha and Perilipin A in methylglyoxal-treated normal Wistar rats. After 48 hours, all rats showed increased fasting glycaemia and insulinemia. However, methylglyoxal-treated rats had higher free fatty acids and triglycerides levels and decreased adiponectinemia, consequent to decreased PPARgamma levels in partially irrigated adipose tissue. Our data show that besides causing vascular dysfunction, glycation further contributes to impaired adipocyte metabolism after a decrease of tissue irrigation, what may hamper metabolic adaptation during tissue expansion.
Acknowledgements
We thank the Serviço de Anatomia Patológica, Coimbra University Hospital, especially Ilda Simões, for the support with histological techniques; the Laboratory of Biostatistics and Medical Mathematics for support in the statistical analysis, especially Dr. João Pereira; and thanks also to Mário Simões from our laboratory for his technical support.
Funding: We thank the Portuguese Foundation for Science and Technology (FCT; project PTDC/SAU-OSM/67498/2006; project Pest-C/SAU/UI3282/2011) and Faculty of Medicine, University of Coimbra.