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Original Article

Preparation and scanning electronic microscopy study of chitosan/polyvinyl (alcohol)-encapsulated crude urease extract

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Pages 639-646 | Received 03 Jun 1996, Accepted 03 Apr 1997, Published online: 26 Jun 2009
 

Abstract

Small capsules of a blended chitosan (QTS)-polyvinyl alcohol (PVA) mixture were prepared by the coacervation salting-out method using sodium sulphate (20%w/v) as a coagulation solution followed by further treatment with a solution containing formic aldehyde (7%w/v)sulphuric acid (20%w/v) and sodium sulphate (25%w/v)., The morphology of the microcapsule wall was observed by scanning electron microscopy (SEM)., The enzyme ureaseas a crude extractwas encapsulated and the cross section of the loaded capsule was observed by SEM., The enzyme extract was fully immobilized and the enzymatic assay showed the presence of the enzyme in an active form with a shift in the pH of maximum activity to a lower pH.

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