Advanced search
Publication Cover
Pathology Volume 36, 2004 - Issue 3
Submit an article Journal homepage
8
Views
0
CrossRef citations to date
0
Altmetric
Microbiology

A PCR method for the identification of methicillin‐resistant Staphylococcus aureus (MRSA) from screening swabs

William W. Hope Microbiology Department, Queensland Health Pathology ServiceCorrespondence[email protected]
,
Anthony P. Morton Infection Management Services, Princess Alexandra Hospital, Brisbane, Queensland, Australia
,
David F. M. Looke Microbiology Department, Queensland Health Pathology Service
,
Jacqueline M. Schooneveldt Infection Management Services, Princess Alexandra Hospital, Brisbane, Queensland, Australia
&
Graeme R. Nimmo Microbiology Department, Queensland Health Pathology Service
Pages 265-268 | Received 24 Jun 2003, Accepted 23 Dec 2003, Published online: 06 Jul 2009

References

  • Bell SM. The CDS disc method of antibiotic sensitivity testing (calibrated dichotomous sensitivity test). Pathology 1975; 7 (Suppl): 1–48.
  • Unal S, Hoskins J, Flokowotsch J, Wu CYE, Preston DA, Skatrud PL. Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. J Clin Microbiol 1992; 30: 1685–91.
  • Murakami Y, Minamide W. PCR Identification of Methicillin-Resistant Staphylococcus aureus. In: Persing DH, Smith TF, Tenover FC, et al., eds. Diagnostic Molecular Microbiology - Principles and Applications. Washington, DC: American Society for Microbiology, 1993; 539–42.
  • Brakstad OG, Aasbakk K, Maeland JA. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J Clin Microbiol 1992; 30: 1654–60.
  • Whitby M, McLaws ML, Berry G. Risk of death from methicillin-resistant Staphylococcus aureus bacteraemia: a meta-analysis. Med J Aust 2001; 175: 264–7.
  • Ayliffe GA, Buckles A, Casewell MW, et al. Revised guidelines for control of MRSA: applying appropriately-based recommendations. J Hosp Infect 1999; 43: 315–6.
  • Coello R, Jimenez J, Garcia M, et al. Prospective study of infection, colonization and carriage of methicillin-resistant Staphylococcus aureus in an outbreak affecting 990 patients. Eur J Clin Microbiol Infect Dis 1994; 13: 74–81.
  • Jonas D, Grundmann H, Hartung D, Daschner FD, Towner U. Evaluation of the mecA femB duplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 1999; 18: 643–7.
  • Jonas D, Speck M, Daschner FD, Grundmann H. Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs. J CIM Microbiol 2002; 40: 1821–3.
  • Oshima T, Miyachi H, Fusegawa H, Masukawa A, Ikeda M, Ando Y. Detection of methicillin-resistant Staphylococcus aureus by in vitro enzymatic amplification of mecA and femA genes. (Japanese.) Rinsho Byori 1993; 41: 773–8.
  • Kearns AM, Seiders PR, Wheeler J, Freeman R, Steward M. Rapid detection of methicillin-resistant staphylococci by multiplex PCR. J Hosp Infect 1999; 43: 33–7.
  • Reischl U, Linde Hi, Metz M, Leppmeier B, Lehn N. Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR. J Clin Microbiol 2000; 38: 2429–33.
  • Domann E, Hossain H, Fussle R, Chakraborty T. Rapid and reliable detection of multiresistent Staphylococcus aureus (MRSA) by multi-plex PCR. (German.) Dtsch Med Wochenschr 2000; 125: 613–8.
  • Jansen GJ, Mooibroek M, Idema J, Harmsen Hi, Welling GW, Degener JE. Rapid identification of bacteria in blood cultures by using fluorescently labeled oligonucleotide probes. J Clin Microbiol 2000; 38: 814–7.
  • Towner KJ, Talbot DC, Curran R, Webster CA, Humphreys H. Development and evaluation of a PCR-based immunoassay for the rapid detection of methicillin-resistant Staphylococcus aureus. J Med Microbiol 1998; 47: 607–13.
  • Krimmer V, Merkert H, von Eiff C, et al. Detection of Staphylococcus aureus and Staphylococcus epidermidis in clinical samples by 16S rRNA-directed in situ hybridization. J Clin Microbiol 1999; 37: 2667–73.
  • Meier KL. Reporting results from studies evaluating diagnostic tests. Clin Microbiol Newslet 2002; 24: 60–3.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.