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Scandinavian Journal of Clinical and Laboratory Investigation Volume 79, 2019 - Issue 3
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Original Article

Telomerase stability and evaluation of real-time telomeric repeat amplification protocol

Aleksandra R. VukašinovićFaculty of Pharmacy, Department of Medical Biochemistry, University of Belgrade, Belgrade, Serbia; View further author information
,
Jelena M. Kotur-StevuljevićFaculty of Pharmacy, Department of Medical Biochemistry, University of Belgrade, Belgrade, Serbia; View further author information
,
Vid MlakarFaculty of Pharmacy, Department of Clinical Biochemistry, University of Ljubljana, Ljubljana, Slovenia; View further author information
,
Miron D. SopićFaculty of Pharmacy, Department of Medical Biochemistry, University of Belgrade, Belgrade, Serbia; View further author information
,
Zorica P. CvetkovićDepartment of Haematology, Clinical Hospital Center Zemun, Belgrade, Serbia; ;Faculty of Medicine, University of Belgrade, Belgrade, Serbia; View further author information
,
Miloš R. PetkovićFaculty of Pharmacy, Department of Organic Chemistry, University of Belgrade, Belgrade, SerbiaView further author information
,
Vesna V. Spasojević-KalimanovskaFaculty of Pharmacy, Department of Medical Biochemistry, University of Belgrade, Belgrade, Serbia; View further author information
,
Nataša B. Bogavac-StanojevićFaculty of Pharmacy, Department of Medical Biochemistry, University of Belgrade, Belgrade, Serbia; View further author information
&
Barbara OstanekFaculty of Pharmacy, Department of Clinical Biochemistry, University of Ljubljana, Ljubljana, Slovenia; Correspondence[email protected]
ORCID Iconhttp://orcid.org/0000-0001-7158-8241View further author information
ORCID Icon show all
Pages 188-193 | Received 02 Nov 2018, Accepted 27 Jan 2019, Published online: 15 Feb 2019

References

  • Gomez DE, Armando GR, Farina HG, et al. Telomere structure and telomerase in health and disease (Review). Int J Oncol. 2012;41:1561–1569.
  • Hiyama K, Hiyama E. Telomere and telomerase in stem cells. Br J Cancer. 2007;96:1020–1024.
  • Saeed H, Iqtedar M. Stem cell function and maintenance – ends that matter: role of telomeres and telomerase. J Biosci. 2013;38:641–649.
  • Wojtyla A, Gladych M, Rubis B. Human telomerase activity regulation. Mol Biol Rep. 2011;38:3339–3349.
  • Chung SS, Wu Y, Okobi Q, et al. Proinflammatory cytokines IL-6 and TNF-αincreased telomerase activity through NF-κB/STAT1/STAT3 activation, and Withaferin A inhibited the signaling in colorectal cancer cells. Mediators Inflamm. 2017;2017:1.
  • Belair CD, Yeager TR, Lopez PM, et al. Telomerase activity: a biomarker of cell proliferation, not malignant transformation. Proc Natl Acad Sci U S A. 1997;94:13677–13682.
  • Vinagre J, Pinto V, Celestino R, et al. Telomerase promoter mutations in cancer: an emerging molecular biomarker? Virchows Arch. 2014;465:119–133.
  • Jäger K, Walter M. Therapeutic targeting of telomerase. Genes. 2016;7:39.
  • Kim N, Piatyszek M, Prowse K, et al. Specific association of human telomerase activity with immortal cells and cancer. Science. 1994;266:2011–2015.
  • Bertorelle R, Rampazzo E, Pucciarelli S, et al. Telomeres, telomerase and colorectal cancer. World J Gastroenterol. 2014;20:1940–1950.
  • Kouroumalis E. Telomerase: a target for colorectal cancer treatment? Cell Cycle. 2015;14:3513–3514.
  • Hou M, Xu D, Bjorkholm M, et al. Real-time quantitative telomeric repeat amplification protocol assay for the detection of telomerase activity. Clin Chem. 2001;47:519–524.
  • Wege H, Chui MS, Le HT, et al. SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity. Nucleic Acids Res. 2003;31:e3.
  • Jodczyk S, Pearson JF, Aitchison A, et al. Telomere length measurement on the Roche LightCycler 480 Platform. Genet Test Mol Biomarkers. 2015;19:63–68.
  • Patel PK, Koti AS, Hosur RV. NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad. Nucleic Acids Res. 1999;27:3836–3843.
  • Gojkovic T, Vladimirov S, Spasojevic-Kalimanovska V, et al. Preanalytical and analytical challenges in gas chromatographic determination of cholesterol synthesis and absorption markers. Clin Chim Acta. 2018;478:74–81.
  • Tsui NB, Ng EK, Lo YM. Stability of endogenous and added RNA in blood specimens, serum, and plasma. Clin Chem. 2002;48:1647–1653.
  • Lança V, Zee RY, Rivera A, et al. Quantitative telomerase activity in circulating human leukocytes: utility of real-time telomeric repeats amplification protocol (RQ-TRAP) in a clinical/epidemiological setting. Clin Chem Lab Med. 2009;47:870–873.
  • Schrader C, Schielke A, Ellerbroek L, et al. PCR inhibitors – occurrence, properties and removal. J Appl Microbiol. 2012;113:1014–1026.
  • Food US Drugs Administration. Guidance for Industry-Bioanalytical Method Validation.
  • Jakupciak JP, Barker PE, Wang W, et al. Preparation and characterization of candidate reference materials for telomerase assays. Clin Chem. 2005;51:1443–1450.
  • O’Callaghan NJ, Fenech M. A quantitative PCR method for measuring absolute telomere length. Biol Proced Online. 2011;13:3.
  • Virno A, Mayol L, Ramos A, et al. Structural insight into the h TERT intron 6 sequence d(GGGGTGAAAGGGG) from 1H-NMR study. Nucleosides Nucleotides Nucleic Acids. 2007;26:1133–1137.

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