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Editorial Article

A rapid one-step method for the isolation of human granulocytes from whole blood

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Pages 235-238 | Received 21 Sep 1981, Accepted 04 Jan 1982, Published online: 17 Mar 2010

References

  • Aguado M. T., Pujol N., Ribiol E., Tura M., Celada A. Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran. J. Immunol. Methods 1980; 32: 41
  • Böyum A. Separation of leucocytes from blood and bone marrow. Scand. J. clin. Lab. Invest. 1968; 21(Suppl. 97)
  • Ferrante A., Thong Y. H. A rapid one-step procedure for purification of mononuclear and polymorphonuclear leucocytes from human blood using a modification of the Hypaque-Ficoll technique. J. Immunol. Methods 1978; 24: 389
  • Ferrante A., Thong Y. H. Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method. J. Immunol. Methods 1980; 36: 109
  • Hjort R., Jonsson A.-K., Vretblad P. A rapid method for purification of human granulocytes using Percoll®. A comparison with dextrans edi-mentation. J. Immunol. Methods 1981; 43: 95
  • Olofsson T., Gartner I., Olsson I. Separation of human bone marrow cells in density gradients of polyvinylpyrrolidone coated silica gel (Percoll). Scand. J. Haematol. 1980; 24: 254
  • Pertoft H., Back O., Lindahl-Kiessling K. Separation of various blood cells in colloidal silica-polyvinylpyrrolidone gradients. Exp. Cell Res. 1968; 50: 355
  • Segal A. W., Fortunato A., Herd T. A rapid single centrifugation step method for the separation of erythrocytes, granulocytes and mononuclear cells on continuous density gradients of Percoll. J. Immunol. Methods 1980; 32: 209
  • Watt S. M., Burgess A. N., Metcalf D. Isolation and surface labeling of murine polymorphonuclear neutrophils. J. cell. Physiol. 1979; 100: 1

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