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Original Article

Human peripheral blood and bone marrow cell separation using density gradient centrifugation on Lymphoprep and Percoll in haematological diseases

Pages 709-714 | Received 26 Feb 1987, Accepted 25 May 1987, Published online: 17 Mar 2010

References

  • Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 1968; 21((Suppl. 97))77–89
  • Ellis W M, Georgiou G M, Roberton D M, Johnson G R. The use of discontinuous Percoll gradients to separate populations of cells from human bone marrow and peripheral blood. J Immunol Meth 1984; 66: 9–16
  • Olofsson T, Gärtner I, Olsson I. Separation of human bone marrow cells in density gradients of polyvinylpyrrolidone coated silica gel (Percoll). Scand J Haematol 1980; 24: 254–62
  • Berkow R L, Dodson R W. Purification and functional evaluation of mature neutrophils from human bone marrow. Blood 1986; 68: 853–60
  • Böyum A. Separation of lymphocytes, granulocytes and monocytes from human blood using iodinated density gradient media. Methods in Enzymology, D DiSabato, J J Langone, H VanVunakis. Academic Press Inc., New York 1984; 108: 88–102
  • Desimone J, Kleve L, Shaeffer J. Isolations of a reticulocyte-rich fraction from normal human blood on Renografin gradients. J Lab Clin Med 1974; 84: 517–24
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  • Macfarlane S D, Tauro G P, Rendall K, Welch J J, Ekert H. Isolation of malignant cells from human bone marrow using a discontinuous Percoll gradient. Am J Hematol 1986; 22: 403–7

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