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Synthetic Communications
An International Journal for Rapid Communication of Synthetic Organic Chemistry
Volume 23, 1993 - Issue 9
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Original Articles

β-Ketoester Reduction by Baker's Yeast Immobilized in Calcium Alginate: An Examination of pH Effects on Enantiospecificity

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Pages 1201-1208 | Received 05 Nov 1992, Published online: 24 Sep 2006

References

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  • The substrates used for assay of the activity of the two enzymes were different and thus it is not possible to say whether the activity in units term really represents the relative concentrations of the two enzymes
  • The results in fig. 1 are reported as average of 3 deter minations using commercial baker's yeast of different batches. The enantiospecificity is reproducible within ± 1%. The yield of the products were 80–85% for reactions at pH 3 to 5 and decreased gradually from 75% to 40% as the pH increased from 6 to 8. At pH 9 to 10 the yield was only 10 to 15%.
  • The product 2a is obtained with e.e. 90% at optimum pH of 3 as compared to 2b which is obtained with e.e. > 99% under optimum conditions. It has been observed by several workers earlier that baker's yeast shows a better enantiospecifity toward compounds possessing phenyl groups1b,1c
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