High Resolution Light Microscopic Evaluation of Boar Semen Quality Sperm Cytoplasmic Droplet Retention in Relationship with Boar Fertility Parameters

REFERENCES

• Aitken, R. (1995) Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev 7:659–668.
   PubMed Web of Science ®Google Scholar
• Akbarsha, M. A., Latha, P. N. L. and Murugaian, P. (2000) Retention of cytoplasmic droplet by rat cauda epididymal spermatozoa after treatment with cytotoxic and xenobiotic agents. J Reprod Fertil 120: 385–390.
   PubMedGoogle Scholar
• Althouse, G. C. (1998) Cytoplasmic droplets on boar sperm cells. Swine Health and Production 6:128.
   Google Scholar
• Amann, R. P., Seidel, G. E. J. and Mortimer, R. G. (2000) Fertilizing potential in vitro of semen from young beef bulls containing a high or low percentage of sperm with a proximal droplet. Theriogenology 54:1499–1515.
   PubMed Web of Science ®Google Scholar
• Andersson, H., Wallgren, M., Rydhmer, L., Lundstrom, K., Andersson, K. and Forsberg, M. (1998) Photoperiodic effects on pubertal maturation of spermatogenesis, pituitary responsiveness to exogenous GnRH, and expression of boar taint in crossbred boars. Anim Reprod Sci 54:121–137.
   Google Scholar
• Bloom, G. and Nicander, L. (1961) On the ultrastructure and development of the protoplasmic droplet of spermatozoa. Zeitschrift für Zellforschung 55:833–844.
   Google Scholar
• Bonet, S. (1990) Immature and aberrant spermatozoa in the ejaculate of Sus domesticus. Anim Reprod Sci 22:67–80.
   Google Scholar
• Borg, K. E., Lunstra, D. D. and Christenson, R. K. (1993) Semen characteristics, testicular size, and reproductive hormone concentrations in mature Duroc, Meishan, Fengjing, and Minzhu boars. Biol Reprod 49:515–521.
   Google Scholar
• Braundmeier, A. G. and Miller, D. J. (2001) Invited review: The search is on: finding accurate molecular markers of male fertility. J Dairy Sci 84:1915–1925.
   PubMed Web of Science ®Google Scholar
• Braundmeier, A. G., Demers, J. M., Shanks, R. D. and Miller, D. J. (2004) The relationship of porcine sperm zona-binding ability to fertility. J Anim Sci 82:452–458.
   Google Scholar
• Clarke, R. N., Bakst, M. R. and Ottinger, M. A. (1984) Morphological changes in chicken and turkey spermatozoa incubated under various conditions. Poult Sci. 63: 801–805.
   PubMed Web of Science ®Google Scholar
• Flowers, W. L. (2002) Increasing fertilization rate of boars: Influence of number and quality of spermatozoa inseminated. J Anim Sci 80 (E. Suppl. 1):E47–E53.
   Google Scholar
• Gadea, J., Selles, E. and Marco, M. (2004) The predictive value of porcine seminal parameters on fertility outcome under commercial conditions. Reprod Domest Anim 39:303–308.
   PubMed Web of Science ®Google Scholar
• Harayama, H., Kanda, S. and Kato, S. (1992) Influence of season on characteristics of epididymal and ejaculated semen in meishan boars. Theriogenology 38:491–500.
   Google Scholar
• Harayama, H., Shibukawa, T., Miyake, M., Kannan, Y. and Kato, S. (1996) Fructose stimulates shedding of cytoplasmic droplets from epididymal boar spermatozoa. Reprod Fertil Dev 8:1039–1043.
   PubMed Web of Science ®Google Scholar
• Huang, S. Y., Kuo, Y. H., Lee, Y. P., Tsou, H. L., Lin, E. C., Ju, C. C. and Lee, W. C. (2000) Association of heat shock protein 70 with semen quality in boars. Anim Reprod Sci 63:231–240.
   Google Scholar
• Huang, S. Y., Chen, M. Y., Lin, E. C., Tsou, H. L., Kuo, Y. H., Ju, C. C. and Lee, W. C. (2002) Effects of single nucleotide polymorphisms in the 5’-flanking region of heat shock protein 70.2 gene on semen quality in boars. Anim Reprod Sci 70:99–109.
   Google Scholar
• Kato, S., Shibukawa, T., Harayama, H. and Kannan, Y. (1996) Timing of shedding and disintegration of cytoplasmic droplets from boar and goat spermatozoa. J Reprod Dev 42:237–241.
   Google Scholar
• Kuster, C. E. and Althouse, G. C. (1999) The fecundity of porcine semen stored for 2 to 6 days in Androhep® and X-cellTM extenders. Theriogenology 52:365–376.
   Web of Science ®Google Scholar
• Kuster, C. E., Hess, R. A. and Althouse, G. C. (2004a) Immunofluorescence reveals ubiquitination of retained distal cytoplasmic droplets on ejaculated porcine spermatozoa. J Androl 25:340–347.
   Google Scholar
• Kuster, C. E., Singer, R. S. and Althouse, G. C. (2004b) Determining sample size for the morphological assessment of sperm. Theriogenology 61:691–703.
   PubMed Web of Science ®Google Scholar
• Larsson, K., Darenius, K. and Johansson, K. (1980) Sperm morphology and in vitro viability in diluted semen in relation to fertility of AI boars. Nord Vet Med 32:533–542.
   Google Scholar
• Lovercamp, K. W., Safranski, T. J., Fischer, K. A., Manandhar, G., Sutovsky, M., Herring, W. and Sutovsky, P. (2007) Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars. Theriogenology 67:704–718.
   PubMed Web of Science ®Google Scholar
• Malmgren, L. and Larsson, K. (1984) Semen quality and fertility after heat stress in boars. Acta Vet Scand 25:425–435.
   Google Scholar
• Petrunkina, A. M., Gehlhaar, R., Drommer, W., Waberski, D. and Töpfer-Petersen, E. (2001) Selective sperm binding to pig oviductal epithelium in vitro. Reproduction 121:889–896.
   Google Scholar
• Popwell, J. M. and Flowers, W. L. (2004) Variability in relationships between semen quality and estimates of in vivo and in vitro fertility in boars. Anim Reprod Sci 81:97–113.
   Google Scholar
• Pruneda, A., Pinart, E., Briz, M., Sancho, S., Garcia-Gil, N., Badia, E., Kádár, E., Bassols, J., Bussalleu, E., Yeste, M. and Bonet, S. (2005) Effects of a high semen-collection frequency on the quality of sperm from ejaculates and from six epididymal regions in boars. Theriogenology 63:2219–2232.
   PubMed Web of Science ®Google Scholar
• Russell, L. D. (1984) Spermiation - the sperm release process: Ultrastructural observations and unresolved issues. In Ultrastructure of Reproduction, Vol. 2. Blerkom, J. V.,, Motta, P. M., (Eds). Boston, , MA:Martinus Nijhoff Publishers, pp. 46–66.
   Google Scholar
• Sancho, S., Pinart, E., Briz, M., Garcia-Gil, N., Badia, E., Bassols, J., Kádár, E., Pruneda, A., Bussalleu, E., Yeste, M., Coll, M. G. and Bonet, S. (2004) Semen quality of postpubertal boars during increasing and decreasing natural photoperiods. Theriogenology 62:1271–1282.
   Google Scholar
• Thundathil, J., Palasz, A. T., Barth, A. D. and Mapletoft, R. J. (2001) The use of in vitro fertilization techniques to investigate the fertilizing ability of bovine sperm with proximal cytoplasmic droplets. Anim Reprod Sci 65:181–192.
   PubMedGoogle Scholar
• Waberski, D., Meding, S., Dirksen, G., Weitze, K. F., Leiding, C. and Hahn, R. (1994) Fertility of long-term-stored boar semen: Influence of extender (Androhep and Kiev), storage time and plasma droplets in the semen. Anim Reprod Sci 36:145–151.
   Google Scholar