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Archives of Andrology
Journal of Reproductive Systems
Volume 53, 2007 - Issue 6
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RESEARCH ARTICLE

Sperm Preparation: DNA Damage by Comet Assay in Normo- and Teratozoospermics

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Pages 325-338 | Received 04 May 2007, Accepted 26 Aug 2007, Published online: 09 Jul 2009

REFERENCES

  • Agarwal, A. and Said, T. M. (2005). Oxidative stress, DNA damage and apoptosis in male infertility a clinical approach. BJU International 95:503–507.
  • Aitken, R. and Krausz, C. (2001). Oxidative stress, DNA damage and the Y chromosome. Reproduction. 122:497–506.
  • Aitken, R. J. (1999). The Amoroso lecture The human spermatozoa-a cell in crisis. J Reprod Fertil 115:1–7.
  • Allamaneni, S. S., Agarwal, A., Rama, S., Ranganathan, P. and Sharma, R. K. (2005). Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa. Asian J Androl 7(1)86–92.
  • American Society for Reproductive Medicine. (2005). Characterization of oxidative stress status by evaluation of reactive oxygen species levels in whole semen and isolated spermatozoa. Fertil Steril 83:800–803.
  • Barroso, G., Morshedi, M. and Oehninger, S. (2000). Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 15:1338–1344.
  • Centola, G. M., Herko, R., Andolina, E. and Weisensel, S. (1998). Comparison of sperm separation methods Effect on recovery, motility, motion parameters, and hyperactivation. Fertil Steril 70:1173–1175.
  • Chatterjee, R., Haines, G. A., Perera, D. M., Goldstone, A. and Morris, I. D. (2000). Testicular and sperm DNA damage after treatment with fludarabine for chronic lymphocytic leukemia. Hum Reprod 15:762–766.
  • Chen, H., Cheung, M. P., Chow, P. H., Cheung, A. L., Liu, W. and O, W. S. (2002). Protection of sperm DNA against oxidative stress in vivo by accessory sex gland secretions in male hamsters. Reproduction 124:491–499.
  • Chohan, K. R., Griffin, J. T., Lafromboise, M., De Jong, C. J. and Carrell, D. T. (2006). Comparison of chromatin assays for DNA fragmentation evaluation in Human sperm. J Androl 27(1)53–59.
  • Donnelly, E. T., O'Connell, M., McClure, N. and Lewis, S. E. M. (2000). Differences in nuclear DNA fragmentation and mitochondrial integrity of semen and prepared human spermatozoa. Hum Reprod 15:1552–1561.
  • Donnelly, E. T., Steel, E. K., McClure, N. and Lewis, S. E. (2001a). Assessment of DNA integrity and morphology of ejaculated sperm from fertile and infertile men before and after cryopreservation. Hum Repro 16:1191–1199.
  • Donnelly, E. T., McClure, N. and Lewis, S. E. (2001b). Cryopreservation of human semen and prepared sperm Effects on motility parameters and DNA integrity. Fertil Steril 76:892–900.
  • Donnelly, E., McClure, N. and Lewis, S. (1999). The effect of ascorbate and alpha-tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide-induced DNA damage in human spermatozoa. Mutagenesis 14:505–512.
  • Duty, S. M., Singh, N. P., Ryan, L., Chen, Z., Lewis, C., Hunag, T. and Hauser, R. (2002). Reliability of the comet assay in cryopreserved human sperm. Hum Reprod 17:1274–1280.
  • Emiliani, S., Van den Bergh, M., Vannin, A. S., Biramane, J., Verdoodt, M. and Englert, Y. (2001). Evidence of reduced single-stranded testicular sperm DNA from obstructive azoospermic men after 3 days of in vitro culture. Hum Reprod 16:1200–1203.
  • Erenpreisa, J., Erenpreiss, J., Freivalds, T., Slaidina, M., Krampe, R., Butikova, J., Ivanov, A. and Pjanova, D. (2003). Toluidine blue test for sperm DNA integrity and elaboration of image cytometry algorithm. Cytometry 52:19–27.
  • Evenson, D. P., Jost, L. K., Marshall, D., Zinaman, M. J., Clegg, E., Purvis, K., De Angelis, P. and Claussen, O. P. (1999). Utility of sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod 14:1039–1049.
  • Evenson, D. P., Jost, L. K., Corzett, M. and Balhorn, R. (2000). Characteristics of human sperm chromatin structure following an episode of influenza and high fever A case study. J Androl 21:739–746.
  • Evenson, D. P., Larson, K. L. and Jost, L. K. (2002). Sperm chromatin structure assay its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with the other techniques. J Androl 23:25–43.
  • Fernandez, J. L., Muriel, L., Rivero, M. T., Goyanes, V., Vazquez, R. and Alvares, J. G. (2003). The sperm chromatin dispersion test A simple method for the determination of sperm DNA fragmentation. J Androl 24:59–66.
  • Golan, R., Shochat, L., Weissenberg, R., Soffer, Y., Marcus, Z., Oschry, Y. and MLeuin, L. M. (1997). Evaluation of chromatin decondensation in human spermatozoa A flow cytometric assay using acridine orange staining. Mol Hum Reprod 3:47–54.
  • Haines, G. A., Marples, B., Daniiel, C. P. and Morris, I. D. (1998). DNA damage in human and mouse sperm after in vitro-irradiation assessed by the comet assay. Adv Exp Med Biol 444:79–93.
  • Haines, G. A., Hendry, J. H., Daniel, C. P. and Morris, I. D. (2002). Increased level of comet-detected sperm DNA damage following in vivo isotopic or X-irradiation of spermatogonia. Mutat Res 495:21–32.
  • Host, E., Svend, L., Jarl, K. A. and Flemming, C. (1999). DNA strand breaks in human sperm cells A comparison between men with normal and oligozoospermic sperm samples. Acta Obstet Gynecol Scand 78:336–339.
  • Host, E., Lindenberg, S. and Smidt-Jensen, S. (1999). DNA strand breaks in human sperm Correlation with fertilization in vitro in oligospermic men and in men with unexplained infertility. Acta Obstet Gynecol Scand 79:189–193.
  • Hughes, C. M., Lewis, S. E., McKelvey-Martin, V. J. and Thompson, W. (1997). Reproducibility of human sperm DNA measurements using alkaline single cell gel electrophoresis assay. Mutat Res 374:261–268.
  • Hughes, C. M., McKelvey-Martin, V. J. and Lewis, S. E. (1999). Human sperm DNA integrity assessed by the Comet and ELISA assay. Mutagenesis 14:71–75.
  • Irvine, D. S., Twig, J. P., Gordon, E. L., Fulton, N., Milne, P. A. and Aitken, R. J. (2000). DNA integrity in human spermatozoa Relationship with semen quality. J Androl 21:33–44.
  • Klaude, M., Eriksson, S., Nygren, J. and Ahnstrom, G. (1996). The comet assay Mechanism and technical considerations. Mutat Res 363:89–96.
  • Kodama, H., Yamaguchi, R., Fukuda, J., Kasai, H. and Tanaka, T. (1997). Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril 65:519–524.
  • Kooij, R. J., de Boer, P., De Vreeden-Elberts, J. M., Ganga, N. A. and Singh, N. (2004). The neutral comet assay detects double strand DNA damage in selected and unselected human spermatozoa of normospermic donors. Int J Androl 27(3)140–146.
  • Kruger, T. F., Acosta, A. A., Simmons, K. F., Kreger, D. O. and Evenson, D. P. (1988). Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 49(1)112–117.
  • Larsen, L., Scheike, T., Jensen, T. K., Bonde, J. P., Ernst, E., Hjollund, N. H., Zhou, Y., Skakkebaek, N. E. and Giwercman, A. (2000). The Danish First Pregnancy Planner Study Team (2000) Computer-assisted semen analysis parameters as predictors for fertility of men from the general population. Hum Reprod 15:1562–1567.
  • Larson, K. L., Brannian, J. D., Singh, N. P., Burbach, J. A., Jost, L. K., Hansen, K. P., Acosta, A. A., Matta, J. F., Oehniger, S. and Morshedi, M. (2001). Chromatin structure in globozoospermia A case study. J Androl 22:424–431.
  • Lopes, S., Sun, J. G., Jurisicova, A., Meriano, J. and Casper, R. F. (1998a). Gamete-specific DNA fragmentation in unfertilized human oocytes after intracytoplasmic sperm injection. Hum Reprod 13:703–708.
  • Lopes, S., Sun, J. G., Jurisicova, A., Meriano, J. and Casper, R. F. (1998b). Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil Steril 69:528–532.
  • Marty, M. S., Singh, N. P., Holsapple, M. P. and Gollapudi, B. B. (1999). Influence of p53 zygosity on select sperm parameters of the mouse. Mutat Res 427:39–45.
  • McKelvey-Martin, V. J., Melia, N., Walsh, I. K., Johnston, S. R., Hughes, C. M., Lewis, S. E. M. and Thompson, W. (1997). Two potential clinical applications of the alkaline single-cell gel electrophoresis assay (1) human bladder washings and transitional cell carcinoma of the bladder and (2) human sperm and male infertility. Mutat Res 375:93–104.
  • McVicar, C. M., McClure, N., Williamson, K., Dalzell, L. H. and Lewis, S. E. (2004) Incidence of Fas positivity and deoxyribonucleic acid double-stranded breaks in human ejaculated sperm. Fertil Steril 81 (Suppl 1)767–774.
  • Morris, L. D., Ilott, S., Dixon, L. and Brison, D. R. (2002). The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Hum Reprod 17(4)990–998.
  • Mortimer, D. (2000). Sperm preparation methods. J Androl 21:357–366.
  • Muriel, L., Segrelles, E., Goyanes, V., GosaÂlvez, J. and Fernandez, J. L. (2004). Structure of human sperm DNA and background damage, analysed by in situ enzymatic treatment and digital image analysis. Mol Hum Reprod 10(3)203–209.
  • O'Connell, M., McClure, N. and Lewis, S. E. (2002). A comparison of mitochondrial and nuclear DNA status in testicular sperm from fertile men and those with obstructive azoospermia. Hum Reprod 17:1571–1577.
  • Olive, P. L., Banath, J. P., Durand, R. E. (1990). Heterogeneity in radiation induced DNA damage and repair in tumor and normal cells using commet assasy. Radiant Res 122:86–94.
  • Ollero, M., Gil-Guzman, E., Lopez, M., Sharma, R., Agarwal, A., Larson, K., Evenson, D., Thomas, A. J. Jr. and Alvarez, J. G. (2001). Characterization of subsets of human spermatozoa at different stages of maturation Implications in the diagnosis of male infertility. Hum Reprod 16:1912–1921.
  • Pasqualotto, F. F., Sharma, R. K., Kobayashi, H., Nelson, D. R., Thomas, A. J. and Agarwal, A. (2001). Oxidative stress in normospermic men undergoing infertility evaluation. Journal of Andrology 22(2)316–322.
  • Ramadan A. Saleh, Ashok Agarwal, Essam A. Nada, Mohamed H. El-Tonsy, Rakesh K. Sharma, Andrew Meyer, David R. Nelson and Anthony J. Thomas (2003). Negative effect of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and mate factor infertility. Fertil Steril 79:1597–1605.
  • Ramos, L., Kleingeld, P., Meulman, E., Van Kooy, R., Kremer, J., Braat, D. and Wetzels, A. (2002). Assessment of DNA fragmentation of spermatozoa that were surgically retrieved from men with obstructive azoospermia. Fertil Steril 77:233–237.
  • Sakkas, D., Urner, F., Bianchi, P. G., Bizzaro, D., Wagner, I., Jaquenoud, N., (1996) Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum Reprod 11:837–843.
  • Sakkas, D., Mariethoz, E., Manicardi, G. (1999a) Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod 4:31–37.
  • Sakkas, D., Mariethoz, E. and St John, J. C. (1999b). Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway. Exp Cell Res 251:350–355.
  • Sakkas, D., Manicardi, G. C., Tomlinson, M., Mandrioli, M., Bizzaro, D., Bianchi, P. G. and Bianchi, U. (2000). The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. Hum Reprod 15:1112–1116.
  • Sakkas, D., Moffatt, O., Manicardi, G. C., Mariethoz, E., Tarozzi, N. and Bizzaro, D. (2002). Nature of DNA damage in ejaculated human spermatozoa and the possible involvement of apoptosis. Biol Reprod 66:1061–1067.
  • Saleh, R. and Agarwal, A. (2002). Oxidative stress and male infertility from research bench to clinical practice. J Androl 23:737–752.
  • Saleh, R., Agarwal, A., Nelson, D., Nada, E., El-Tonsy, M., Alvarz, J. G., Thomas, A. J. Jr. and Sharma, R. (2002a). Increased sperm nuclear DNA damage in normozoospermic infertile men A prospective study. Fertil Steril 78:313–318.
  • Saleh, R., Agarwal, A., Sharma, R., Nelson, D. and Thomas, A. J. Jr. (2002b). Effect of cigarette smoking on levels of seminal oxidative stress in infertile men A prospective study. Fertil Steril 78:491–499.
  • Saleh, R. A., Agarwal, A., Nada, E. A., El-Tonsy, M. H., Sharma, R. K., Meyer, A., (2003) Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril 79(Suppl 3)1597–1605.
  • Sharma, R. K. and Agarwal, A. (1996). Sperm quality improvement in cryopreserved human semen. J Urol 156:1008–1012.
  • Sharma, R. K., Said, T. and Agarwal, A. (2004). Sperm DNA damage and its clinical relevance in assessing reproductive outcome. Asian J Androl 6:139–148.
  • Sierens, J., Hartley, J. A., Campbell, M. J., Leathem, A. J. and Woodside, J. V. (2002). In vitro isoflavone supplementation reduces hydrogen peroxide-induced DNA damage in sperm. Teratog Carcinog Mutagen 22:227–234.
  • Singh, N. P., Danner, D. B., Tice, R. R., McCoy, M. T., Collins, G. D. and Schneider, E. L. (1989). Abundant alkali-sensitive sites DNA of human and mouse sperm. Exp Cell Res 184:461–470.
  • Singh, N. P. and Stephens, R. E. (1998). X-ray-induced DNA double-strand breaks in human sperm. Mutagenesis 13:75–79.
  • Spano, M., Bonde, J. P., Hjollund, H. I., Kolstad, H. A., Cordelli, E. and Leter, G. (2000). Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study team. Fertil Steril 73:43–50.
  • Steele, E. K., McClure, N., Maxwell, R. J. and Lewis, S. E. (1999). A comparison of DNA damage in testicular and proximal epididymal spermatozoa in obstructive azoospermia. Mol Hum Reprod 5:831–835.
  • Sun, J. G., Jurisicova, A. and Casper, R. F. (1997). Detection of deoxyribonucleic acid fragmentation in human sperm Correlation with fertilization in vitro. Biol Reprod 56:602–607.
  • Tamer, M. S., Agarwal, A., Sharma, R. K., Thomas, A. J. and Sikka, S. C. (2005). Impact of sperm morphology on DNA damage caused by oxidative stress induced by nicotinamide adenine dinucleotide phosphate. Fertility and Sterility 83(1)95–103.
  • Tesarik, J., Greco, E. and Mendoza, C. (2001). Assisted reproduction with in-vitro-cultured testicular spermatozoa in cases of severe germ cell apoptosis A pilot study. Human Reproduction 16(12)2640–2645.
  • Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, C. and Sasaki, Y. E. (2000). Single cell gel/ comet assay guidelines for in vitro and in vivo genetic toxicology testing. Envion Mol Mutagen 35:206–221.
  • Tomlinson, M., Moffatt, O., Manicardi, G., Bizzaro, D., Afnan, M. and Sakkas, D. (2001). Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation Implications for assisted conception. Hum Reprod 16:2160–2165.
  • Twigg, J., Irvine, D. and Aitken, J. (1998a). Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at intracytoplasmic sperm injection. Hum Reprod 13:1864–1871.
  • Twigg, J., Irvine, D. S., Houston, P., Fulton, N., Michael, L. and Aitken, R. J. (1998b). Iatrogenic DNA damage induced in human spematozoa during sperm preparation Protective significance of seminal plasma. Mol Hum Reprod 4:439–445.
  • vonSonntag, C. (1987) The chemical basis of radiation biology. London Taylor & Francis, pp 210–213.
  • Wang, X., Sharma, R. K., Sikka, S. C., Thomas, A. J. Jr., Falcone, T. and Agarwal, A. (2003). Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility. Fertil Steril 80:531–535.
  • Ward, W. S. and Coffey, D. S. (1991). DNA packaging and organization in mammalian sperm Comparison with somatic cells. Biol Reprod 44:569–574.
  • World Health Organization. (1999) WHO laboratory manual for the examination of human semen and semen-cervical mucus interactions. 4th ed. United Kingdom Cambridge University Press Cambridge.
  • Younglai, E. V., Holt, D., Brown, P., Jurisicova, A. and Casper, R. F. (2001). Sperm swim-up technique and DNA fragmentation. Hum Reprod 16:1950–1953.
  • Zini, A., Finelli, A., Phang, D. and Jarvi, K. (2000). Infuence of semen processing technique on human sperm DNA integrity. Urology 56:1081–1084.
  • Zini, A., Kamal, K., Phang, D., Willis, J. and Jarvi, K. (2001a). Biologic variability of sperm DNA denaturation in infertile men. Urology 58:258–261.
  • Zini, A., Bielecki, R., Phang, D. and Zenzes, M. T. (2001b). Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril 75:674–677.

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